Thin Layer Chromatography

SCIENTIFIC CONCEPTS/PROCESSES

• Develop understanding of thin layer chromatography
• Establish Rf values to establish standards
• Use Rf standards to identify “unknowns”
This inexpensive, simple, and very accurate Lab-Aid illustrates the principles of Thin Layer Chromatography (TLC). The lab techniques involve silica-gel coated plastic slides, indicators, developers and chromatography chambers. Each student runs chromatograms of five different sample dye solutions in order to establish Rf (Rate of flow) standards. They also run chromatograms of two “unknowns,” which are identified for the teacher in the instruction booklet.

This approach gives students an opportunity to appreciate the usefulness of TLC. TLC, which is similar and complementary to paper chromatography, can also be employed to determine the purity of a system.

This kit is complete for 24 students and can be performed in a single lab period.

Contents List

• 1 Teachers Guide with MSDS
• 24 Student Worksheets and Guides
• 4 btls TLC solvent
• 12 Developing Chambers with caps
• 72 TLC layers
• 2 vials capillary applicators

Drop Control Bottles:

• 1 Indigo Carmine
• 1 Methyl Blue
• 1 Sudan IV