AP Biology Investigation #8: Biotechnology: Bacterial Transformation

Students will genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance. They will then examine the number of resistant bacteria to determine the efficiency of the transformation procedure.

This kit contains enough materials for 8 groups. Teacher’s manual and Student Study Guide copymasters are included.

Meets AP Science Practices 1, 3, 5, 6, and 7, and Big Idea 3.

Kit Includes:

• Sterile Calcium Chloride 0.05M - 30ml
• Sterile Volumetric Pipet - 16 pcs
• Pipet Bulb - 8 pcs
• Micropipet w/Plunger 10ul - 8 pcs
• Microcentrifuge Tube 1.5ml - 16 pcs
• Sterile Inoculating Loop - 8 pcs
• Petri Dish - 40 pcs
• Luria Agar 200ml - 4 btl
• E. Coli Culture (freeze dried)
• Luria Broth 9ml Tube
• Kit Contains coupon for perishable materials. Redeem by fax, phone or e-mail.

• 0.02g Ampicillin - 2 btls
• 1000 ng pUC 19 Plasmid
• Tryptic Soy Broth Tube 9ml
• Tryptic Soy Agar Slants 6ml
• Pipet, Sterile, Disposable

Materials Needed but not Supplied:

• Large beaker
• Hot plate or waterbath
• Microwave (not necessary if melting agar in a waterbath)
• Markers
• Microfuge tube racks
• Container of ice

• Incubator (37 Celsius)

DOT: Non-Regulated

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