AP Biology Investigation #4: Diffusion and Osmosis

Students will study the movement of water and nutrients across a cell membrane and observe osmosis in living tissue. They will then investigate the relationship between surface area and volume as it relates to cells and diffusion. They will also examine the concept of molarity and how it relates to osmotic potential and the movement of water. Students will also be able to explain how cell size and shape affect the overall rate of nutrient intake and water elimination. Students will also use plant tissue to determine the molarities of unknown solutions based on the direction and degree of water movement.

This kit contains enough materials for 8 groups. Teacher’s manual and Student Study Guide copymasters are included.

Meets AP Science Practices 2, 4, and 5, and Big Idea 2.

Kit Includes:

• 45g Agar
• 15ml Bromothymol blue concentrate
• Dialysis tubing - 40 pcs.
• Hydrochloric acid 2.0M - 25ml x 2 Bottles
• Plastic cups, 7oz - 40 pcs.
• Plastic cups 5 oz - 8 pcs.
• Agar block casting trays - 8 pcs.
• Microscope slides - pkg/72
• Coverslips
• Plastic knives - 8 pcs.
• Cork borer
• Plastic stirrers - 8 pcs.
• Metric rulers - 8 pcs.
• Sucrose QuickSolution (to make 1L of 1.0M solution)
• Sodium chloride QuickSolution (to make 1L of 1.0M solution)
• Glucose QuickSolution (to make 1L of 1.0M solution)
• Ovalbumin QuickSolution (to make 1L of 5.0% solution)
• Food coloring set (red, blue, yellow, green)
• Sucrose QuickSolution set to make:
• 1L of 0.2M solution (Solution #3)
• 1L of 0.4M solution (Solution #2)
• 1L of 0.6M solution (Solution #5)
• 1L of 0.8M solution (Solution #1)
• 1L of 1.0M solution (Solution #4)
• 1L distilled water (Solution #6)

Materials Needed but not Supplied:

• Paper towels
• Distilled or deionized water
• Electronic balance
• Large onion
• Fine-point forceps/tweezers
• Microscopes
• Potatoes, sweet potatoes, or yams

Please note that the pictures may show items not included in this kit, the pictures are there to show kit functionality. Please refer to the product details above for more information on the contents of this kit.

DOT: Small Quantity Exemption 173.4 This Package Conforms to 49 CFR 173.4 for domestic highway or rail transport only

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