AP Biology Investigation #13: Enzyme Activity

Students will learn the nature and specificity of enzyme-catalyzed reactions. Students will then use an extract of turnips to examine the reaction between hydrogen peroxide and the enzyme peroxidase while testing one or more factors that influence the rate of enzyme reactions.In this lab students will develop data collection strategies and analyze their results.

Teachers manual and Student Study Guide copymasters are included.

This kit contains enough materials for 8 groups.

Meets AP Science Practices 4, 5, 6, and 7, and Big Idea 4.

Kit Includes:

• 1.5mL Guaiacol concentrate 50ml 1.0% Hydrogen peroxide
• 100ml pH 3 Buffer
• 100ml pH 5 Buffer
• 100ml pH 6 Buffer
• 100ml pH 7 Buffer
• 100ml pH 8 Buffer
• 100ml pH 10 Buffer

Materials Needed but not Supplied:

• Test tube 16x150mm
• 8 Test tube racks
• 16 Graduated pipettes 1ml
• 8 Graduated pipettes 5ml
• 16 Graduated pipettes 10ml
• 8 pipette pumps or pipette bulbs
• 8 Timers/ Stop watches
• Parafilm or Similar
• Distilled or deionized water

• Spectrophotometer

DOT: Non-Regulated

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