AP Biology Investigation #11: Transpiration

Students will study the process of transpiration/transpiration pull and the role it plays in water/nutrient movement in plants. They will study the role of stomata in relation to the transpirationprocess using a potometer to examine the rate of transpiration in a bean seedling under a select set of environmental conditions. They will also examine the location/density of stomata from a leaf surface by preparing a stomatal peel. Lastly students will design and conduct an experiment to show how altering an environmental condition may affect the rate of transpiration.

This kit contains enough materials for 32 students in 8 groups.

Meets AP Science Practices 1, 2, 4, 6, and 7, and Big Idea 4.

Kit Includes:

• Clear tubing, 12 Inch - 8 pcs
• Tubing clamps - 8 pcs
• Pipettes, 1ml - 8pcs
• Syringes, 10ml - 8 pcs
• Planting trays 6 cell - 8 pcs
• Potting soil - 1 bag
• Bean seeds - 1 pkg
• Petroleum jelly - 1 tube
• Nail polish - 1 btl
• Microscope slides - 1 pk

Materials Needed but not Supplied:

• Calculators
• Microscopes
• Distilled or deionized water
• Clear cellophane tape
• Razor blades or scalpels
• Laboratory stands w/clamps
• Light source
• Large shallow trays/containers
• Miscellaneous plant leaves

• Fan
• Large plastic bags
• Thermometers
• Spray bottles

DOT: Small Quantity Exemption 173.4 This Package Conforms to 49 CFR 173.4 for domestic highway or rail transport only

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